Gas chromatographic profiling of the biocatalytic conversion of sclareol to ambradiol by <i>Hyphozyma roseoniger</i>

Hyphozyma roseoniger is a filamentous yeast used for its biocatalytic ability to convert sclareol, plant diterpenoid ambradiol, an intermediate ambrafuran, which sought-after fixative in the perfume industry. Metabolite profiling suitable investigative tool dissect biochemical steps involved reaction by H. from sclareol ambradiol. suspensions were grown batch culture simulate growth bioreactor over 14-day period. Cells harvested at stipulated days (phases of growth) using ethyl acetate extraction procedure. The progress bioconversion was monitored gas chromatography with flame ionisation detection (GC–FID) profiling. Here, decreasing concentrations concomitant increasing ambradiol as product and sclareolide putative intermediate, recorded. While presence unidentified peaks noted GC chromatograms, no previously unknown intermediates could be identified sequence